hepatocellular carcinoma cell lines hep3b  (Procell Inc)

Journal: Translational Gastroenterology and Hepatology

Article Title: Leptin-dependent fat accumulation triggers autophagy in metabolic dysfunction-associated steatohepatitis model

doi: 10.21037/tgh-25-17

Figure Lengend Snippet: Real time monitoring of the autophagy process in untreated Hep3B cells. The cells were incubated for 48 h with complete growth medium. Hep3B cells were previously transfected with a plasmid expressing GFP-mRFP-LC3B. Green fluorescence is a marker of early autophagosomes. Instead, the red fluorescence remained stable after the fusion of the autophagosomes with lysosomes thus highlighting the late phase of autophagy.

Article Snippet: The human hepatoblastoma cell line HepG2 (ACC180, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany), the human hepatocellular carcinoma cell line Hep3B (ACC93, DSMZ) and the human HSC line LX-2, a kindly gift from Scott Friedmann (Icahn School of Medicine at Mount Sinai), were grown in DMEM (Gibco, Paisley, UK) supplemented with 10% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) (11548876, Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques:

Journal: Translational Gastroenterology and Hepatology

Article Title: Leptin-dependent fat accumulation triggers autophagy in metabolic dysfunction-associated steatohepatitis model

doi: 10.21037/tgh-25-17

Figure Lengend Snippet: Real time monitoring of the autophagy process in Hep3B cells treated for 48 h with 2 mM oleic acid. Hep3B cells were previously transfected with a plasmid expressing GFP-mRFP-LC3B. Green fluorescence is a marker of early autophagosomes. Instead, the red fluorescence remained stable after the fusion of the autophagosomes with lysosomes thus highlighting the late phase of autophagy.

Article Snippet: The human hepatoblastoma cell line HepG2 (ACC180, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany), the human hepatocellular carcinoma cell line Hep3B (ACC93, DSMZ) and the human HSC line LX-2, a kindly gift from Scott Friedmann (Icahn School of Medicine at Mount Sinai), were grown in DMEM (Gibco, Paisley, UK) supplemented with 10% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) (11548876, Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques:

Journal: Translational Gastroenterology and Hepatology

Article Title: Leptin-dependent fat accumulation triggers autophagy in metabolic dysfunction-associated steatohepatitis model

doi: 10.21037/tgh-25-17

Figure Lengend Snippet: Detection of the autophagy markers in liver cancer cells. (A) RT-qPCR detection of BECN1, MAP1LC3B, SQSTM1, UVRAG, TFEB, PRKAA1_1, and PRKAA2_1 in HepG2 cells after the administration of 2 mM OA, 100 pM BAF, 2 mM caffeine and their combination. The target expression was normalized to GAPDH. Data are presented as means ± SEM of treated vs. untreated cells from three independent experiments. (B,C) Western blot detection and densitometric quantification of the protein level of Beclin1, LC3B-I, LC3B-II, UVRAG, p62, AMPK-α and P-AMPK-α. Beta-actin was detected as equal loading control and used for the further densitometric normalization of the protein level of the target proteins. The error bars of the densitometric graph represent the SEM of experiments performed in triplicates. (D) Micrographs of MAP1LC3B-GFP-RFP stably transfected Hep3B cells after the administration of 2 mM OA (scale bar =300 µm). The cells were live monitored for up to 48 h (please refer to the ). *, P<0.05 by 2-way ANOVA with Dunnett’s test (see Appendix 1 for detailed statistical analysis). ANOVA, analysis of variance; OA, oleic acid; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SEM, standard error mean.

Article Snippet: The human hepatoblastoma cell line HepG2 (ACC180, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany), the human hepatocellular carcinoma cell line Hep3B (ACC93, DSMZ) and the human HSC line LX-2, a kindly gift from Scott Friedmann (Icahn School of Medicine at Mount Sinai), were grown in DMEM (Gibco, Paisley, UK) supplemented with 10% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) (11548876, Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Control, Stable Transfection, Transfection, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Translational Gastroenterology and Hepatology

Article Title: Leptin-dependent fat accumulation triggers autophagy in metabolic dysfunction-associated steatohepatitis model

doi: 10.21037/tgh-25-17

Figure Lengend Snippet: Autophagosome maturation monitoring in Hep3B treated with oleic acid. Intracellular lipid accumulation in HepG2 cells treated for 24 h with 500 ng/mL of rhTGF-β, 2 mM oleic acid, 100 pM bafilomycin, 2 mM caffeine and their combination. The lipids were stained with Oil Red O. Magnification: 100× (A,C) and 200× (B,D).

Article Snippet: The human hepatoblastoma cell line HepG2 (ACC180, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany), the human hepatocellular carcinoma cell line Hep3B (ACC93, DSMZ) and the human HSC line LX-2, a kindly gift from Scott Friedmann (Icahn School of Medicine at Mount Sinai), were grown in DMEM (Gibco, Paisley, UK) supplemented with 10% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) (11548876, Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques: Staining

Journal: Journal of Hepatocellular Carcinoma

Article Title: Coilin Affects the Prognosis of Hepatocellular Carcinoma Through Cell Cycle and Apoptosis

doi: 10.2147/JHC.S500119

Figure Lengend Snippet: Expression of COIL in HCC cell lines and HCC tissue from hospital. The expression of COIL in liver cancer cell lines huh7, hepG2 and hep3B was higher than that in L02 by immunohistochemistry ( A ) and Western blotting ( B ). ( C ) The relative expression of COIL in HCC was significantly higher than that in normal liver tissue by qRT-PCR (***p value < 0.001). ( D ) The expression of COIL in HCC tissue was higher than that in normal liver tissue. ( E, F ) Representative immunohistochemistry results of low or high COIL expression. ( G ) High COIL expression was associated with poor overall survival.

Article Snippet: The hepatocellular carcinoma cell lines HepG2, Hep3B, and Huh7 were purchased from Cyagen Biosciences (Guangzhou) Co., Ltd.

Techniques: Expressing, Immunohistochemistry, Western Blot, Quantitative RT-PCR

Journal: Journal of Hepatocellular Carcinoma

Article Title: Coilin Affects the Prognosis of Hepatocellular Carcinoma Through Cell Cycle and Apoptosis

doi: 10.2147/JHC.S500119

Figure Lengend Snippet: ( A ) Knockdown of COIL in HepG2, Huh7, and Hep3B (***p value < 0.001). ( B ) The effect of COIL on cell invasion ability was determined by Transwell invasion assay. ( C ) The effect of COIL on cell proliferation was determined by CCK-8 assay. ( D ) The cell migration was evaluated by scratch wound healing dynamic test. Western blotting detected the expression of apoptosis ( E ) and cell cycle-related genes ( F ).

Article Snippet: The hepatocellular carcinoma cell lines HepG2, Hep3B, and Huh7 were purchased from Cyagen Biosciences (Guangzhou) Co., Ltd.

Techniques: Knockdown, Transwell Invasion Assay, CCK-8 Assay, Migration, Western Blot, Expressing

Journal: BioImpacts : BI

Article Title: Assessment of the biocompatibility and biodistribution of fluorescent oleic acid capped ZnSe/CdS/ core shell quantum dots after intravenous injection in Balb/c mice

doi: 10.34172/bi.30467

Figure Lengend Snippet: Enumeration of the percent cytotoxicity in HEK- 293, Hep3B and EAC cell line at various time points

Article Snippet: Mouse Ehrlich Ascitic Carcinoma (EAC), Human Embryonic Kidney (HEK-293), Human Hepatocellular Carcinoma- Hep3B cell lines were procured from National Centre for Cell Sciences (NCCS), India.

Techniques: